JPBIO (Jurnal Pendidikan Biologi)
type: channel
description: Journal title JPBIO (Jurnal Pendidikan Biologi) Initials JBIO Online Since 2016 Grade Accredited Sinta 3 Journal; decree No. 85/M/KPT/2020 Frequency 2 Issues per Year (April and November) Online ISSN 2540-802X Editor-in-chief Yakobus Bustami (Scopus ID: 57194697472) Managing Editor Anyan (Sinta ID: 5999449) Publisher Department of Research and Community Service of STKIP Persada Khatulistiwa Sintang DOI Prefix 10.31932 by <img src="[binary]" alt="" /> Indexed Sinta|Â Google Schoolar|Â Index Copernicus|Garuda|Â BASE|Dimensions JPBIO (Jurnal Pendidikan Biologi) is a scientific journal published by Department of Research and Community Service of STKIP Persada Khatulistiwa Sintang with e-ISSN 2540-802X receiving manuscripts contribution that have never been published or sent to other journals related biology and biology education with the topics: (1) teaching and learning; (2) case study, lesson study, classroom action research, experiments, and others; (3) education curriculum; (4) learning evaluation; (5) learning media; (6) development of teaching materials; (7) school management or laboratory management; (8) problems / trends in the field of education; and (9) biological sciences. JPBIO (Jurnal Pendidikan Biologi) was published twice a year, on April and November and at least 5 articles per issue. This journal written in English. All submitted manuscripts will be initially reviewed by editors and are then evaluated by two reviewers through the double-blind review process. National accredited Sinta 3 by the Ministry of Research-Technology and Higher Education Republic of Indonesia with decree Number 85/M/KPT/2020.
publisher: STKIP Persada Khatulistiwa Sintang
language: en-US
publication name: JPBIO (Jurnal Pendidikan Biologi)
issn: 2540-802X
copyright: Authors who publish on JPBIO (Jurnal Pendidikan Biologi) agree to the following terms: The all articles published on JPBIO (Jurnal Pendidikan Biologi), authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work. JPBIO (Jurnal Pendidikan Biologi) has no responsibility for claims or claims to be filed by third parties due to copyright infringement and make every effort to ensure that no wrong or misleading data, opinions, or statements be published in the journal. The authors fully responsible for the content of articles and advertisements published in the journal JPBIO (Jurnal Pendidikan Biologi), authors accept and reassure you to transfer all our rights on the article, including processing, reproduction, representation, printing, publishing, distribution, and electronic transmission, to be used by JPBIO (Jurnal Pendidikan Biologi) officials for unlimited use. At the time of submission of the manuscript, the authors asked to complete the 'Copyright Transfer Form' and should be signed electronically. The Copyright Transfer Form can be downloaded here.
items: Enhancing high-order thinking abilities through disc socio-scientific issues discussion on the virus concept
items: Influence of guided inquiry on science literacy in biodiversity with islamic values
items: Isolation and characterization of phosphate-solubilizing bacteria in land managed with the dusung system
items: Advances in diabetes intervention: Targeting hormonal regulation, hIAPP, glucose transport, and key enzymatic pathways
items: Remap NHT learning model integrates scientific literacy and critical thinking to enhance students creative thinking
items: Study of dogania subplana aclimatization in the turtle learning center university of bengkulu
items: Climate change literacy ability profile in junior high school students in ogan ilir regency
items: Factors influencing green product purchase of adiwiyata and non-adiwiyata high school students in south jakarta
items: Supporting sustainable development goals 4 through ethnoscience: Development of a scientific magazine on madurese shrimp paste
items: Analysis of students’ science process skills in low plant botany online learning through telegram
items: Integrating STEM education and socio-scientific issues: Impacts on student creativity
items: Reduction lead metal in process marinating local food fish pendap using lemon juice (citrus limon)
items: The influence of the discovery learning model with differentiated learning on the HOTS learning outcomes
items: The effect of nearpod-assisted problem-based learning on environmental literacy
items: The implementation of quantum learning strategies in ecology materials to enhance students' creative thinking skills
items: Community structure and exploitation pressure of giant clam in dedap island-abang waters batam
items: Reducing academic burnout biology among boarding school students through LIVE (liveworksheet and videos)
items: Effectiveness of a project-based learning digital booklet developed with the 4D model
items: Development of augmented reality-based flashcard learning media for teaching leaf types
items: The implementation of discovery learning to improve scientific literacy in biology learning of protists
items: Correlation of cognitive learning outcomes and creativity in PjBL with culturally responsive teaching
items: Climate change e-module based on socio-scientific issues teaching and learning: A validity and practicality study
items: Problem-based learning quartet cards development assisted by QR code: Critical thinking on environmental change
items: Exploration of lactic acid bacteria from husk and black rice (Oryza sativa L) of toraja origin
items: E-module practicum of plant tissue culture with augmented reality to enhance lateral thinking and digital literacy
items: Variation of morphological response of cayenne pepper cultivars to phenoxaprop-p-ethyl herbicide
items: Effect honje forest fruit juice administration on hemoglobin levels following mercury chloride treatment in mice
items: BioDiv-Web: Integrating structured project-based learning with citizen science to enhance critical thinking and environmental literacy
items: Ethnobotany of spice plants among the community of emparu baru village, dedai district, sintang regency

Enhancing high-order thinking abilities through disc socio-scientific issues discussion on the virus concept
type: item
description: Based on national and international assessments, Indonesian students' higher-order thinking is inadequate, highlighting the need for effective learning. This study aimed to explore the impact of discussion of a socio-scientific issue on the development of higher-order thinking related to the concept of viruses. A quasi-experimental design used a nonequivalent pretest and posttest control group framework. The experimental group engaged in a socioscientific issue discussion model comprising the stages of Polemic, Exploration, Action, and Conclusion (PEAK). In contrast, the control group was instructed with a constructivist learning approach with discussion, students' presentations, and teacher explanations. The experimental group included 26 males and 46 females, while the control group had 30 males and 41 females. The findings showed that the experimental group's average pretest score was 55.1, while the control group's was 60,1. After the intervention, the experimental group achieved a posttest average of 73,3, compared to 69 for the control group. The N-gain test results demonstrated higher average gain in the experimental group (x-gain 0,5) than the control group (x-gain 0,2). Additionally, the Mann-Whitney U test indicated a significant effect of the socioscientific issue discussion learning model on students' HOTS. This model offers a promising alternative to enhance students' high-level thinking.
creator: Adelia Siti Nur Apriliani
creator: Yanti Herlanti
rights: Copyright (c) 2025 Adelia Siti Nur Apriliani
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 191
ending page: 203
doi: 10.31932/jpbio.v10i2.4700

Influence of guided inquiry on science literacy in biodiversity with islamic values
type: item
description: The literacy level of science students in Indonesia is classified as low, especially in integrated with biology and Islamic values. This is influenced by ongoing learning, teacher-centered, limited implementation, learning is active, and a lack of integration of Islamic values in learning science in the Madrasah. Therefore, this research was conducted to now the influence of learning models inquiry guided by the ability of literacy science student class X on the material diversity and biologically integrated Islamic values at MAN 21 Jakarta. Research: This used quasi- quasi-experimental design with a pretest-posttest control group design. Inquiry model guided is an involved learning model students in a way active scientific investigation with teacher guidance through worksheets Students (LKPD), teaching modules, and resources relevant to learning, as well as blending Islamic values to increase student understanding in a way. Instrument study in the form of a PISA-based open-constructed response test. The results of the analysis show there is a significant difference between class experiments and class controls after application of the inquiry-guided learning model. This shows that learning model inquiry-guided is effective in increasing literacy in science students.
creator: Chaira Dela Adela
creator: Zulfiani Zulfiani
creator: Solihin Solihin
rights: Copyright (c) 2025 Chaira Dela Adela, Zulfiani Zulfiani, Solihin S
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 204
ending page: 219
doi: 10.31932/jpbio.v10i2.4800

Isolation and characterization of phosphate-solubilizing bacteria in land managed with the dusung system
type: item
description: Soil microorganisms play a crucial role in nutrient cycling and soil fertility, particularly through their ability to solubilize insoluble phosphate compounds. This study aims to isolate and characterize phosphate-solubilizing bacteria (PSB) on land managed under the dusung agroforestry system. Soil samples were collected from five types of land use: forests, mixed orchards, clove hamlets, nutmeg orchards, and shrubs. PSB isolation was performed using Pikovskaya medium with the spread plate method, followed by characterization of colony morphology (shape, edge, color, elevation) and Gram staining. The results showed that the highest PSB population occurred in forest land (8.3 × 10? CFU/ml), followed by mixed orchards (6.2–6.7 × 10? CFU/ml), clove orchards (5.3–5.4 × 10? CFU/ml), nutmeg orchards (3.2–5.6 × 10? CFU/ml), and the lowest in shrubs (7.1 × 10³ CFU/ml). The characteristics of PSB varied, with isolates from forests and nutmeg orchards predominantly consisting of coccus-shaped Gram-positive bacteria, while those from mixed and clove orchards were dominated by bacillus-shaped Gram-positive bacteria. The population and diversity of PSBs were influenced by the availability of organic matter, soil pH, and land management practices. These findings highlight the ecological importance of the dusung system in sustaining soil fertility and promoting ecosystem resilience.
creator: Morgan Ohiwal
creator: Rendy Marasabessy
creator: Melda Yunita
creator: Sukmawati Sukmawati
creator: Mariyatul Qibtiyah
rights: Copyright (c) 2025 Morgan Ohiwal, Melda Yunita
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 220
ending page: 229
doi: 10.31932/jpbio.v10i2.4821

Advances in diabetes intervention: Targeting hormonal regulation, hIAPP, glucose transport, and key enzymatic pathways
type: item
description: Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia resulting from impaired insulin secretion or action. This condition remains a leading cause of global morbidity and mortality and is closely associated with various metabolic complications and cancer risk. This study aimed to identify potential molecular targets and hormonal regulatory mechanisms involved in diabetes management. A systematic review was conducted by searching indexed scientific articles in databases such as ScienceDirect and Google Scholar using the keywords “diabetes,” “molecular targets,” “insulin sensitivity,” and “hormonal regulation.” The analysis identified six major molecular mechanisms contributing to improved insulin action and glucose homeostasis, involving targets such as human islet amyloid polypeptide (hIAPP), ?-glucosidase, dipeptidyl peptidase-4 (DPP-4), sodium-glucose co-transporter-2 (SGLT2), glucose transporter type 4 (GLUT4) translocation, RNA-binding proteins (RBPs), and key enzymatic pathways associated with glucose metabolism. The development of therapeutic approaches directed toward these pathways could significantly enhance diabetes control, improve insulin sensitivity, and prevent long-term complications.
creator: Mulia Safrida Sari
creator: Lilla Puji Lestari
creator: Meri M. Imun
creator: Cut Sriyanti
creator: Nurlaili Ramli
rights: Copyright (c) 2025 Mulia Safrida Sari
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 230
ending page: 249
doi: 10.31932/jpbio.v10i2.4850

Remap NHT learning model integrates scientific literacy and critical thinking to enhance students creative thinking
type: item
description: Students' scientific literacy skills help them analyze and evaluate each scientific subject. It is predicted that scientific literacy skills will help students improve their communication abilities as well. This study evaluated the correlation between scientific literacy and Critical thinking skills in developing creative thinking skills using the Remap-NHT learning model. The study was quasi-experimental since it employed a non-equivalent pretest-post-test control group design and included 122 eleventh-grade students at SMAN 4 Soppeng during the 2023/2024 academic year. The participants were divided into three groups. An essay test assessed every variable. The findings indicated that (1) there was a significant correlation between scientific literacy skills and creative thinking skills; (2) there was a significant correlation between Critical thinking skills and creative thinking skills; and (3) there was a significant correlation between scientific literacy skills and Critical thinking skills. The regression equation showed Y= 0,228*X1+0.995*X2+21,645. Based on the findings, it can be concluded that Remap-NHT can be utilized to improve students' scientific literacy, communication, and creative thinking skills, all of which are demonstrated to be connected.
creator: Ferry Irawan
creator: Nur Zakiyah R
rights: Copyright (c) 2025 Ferry Irawan
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 250
ending page: 260
doi: 10.31932/jpbio.v10i2.4906

Study of dogania subplana aclimatization in the turtle learning center university of bengkulu
type: item
description: The forest softshell turtle (Dogania subplana) is a species increasingly threatened by habitat destruction and overexploitation. This study aims to identify the acclimatization process of D. subplana in an artificial environment, specifically, an aquarium at the Turtle Learning Center (TLC). The research was conducted over 30 days from July to August 2024. Observations were made on daily behavior, body temperature, and health condition, individual biometric measurements, and environmental (abiotic) parameters. The results showed that daily behaviors such as feeding, basking, and hiding occurred regularly, indicating the species' ability to adapt to a new environment. The turtles experienced an increase in body weight, while growth in carapace length and width was very slow. The body temperature of D. subplana varied in accordance with the surrounding environmental temperature, reflecting its dependence on ambient conditions. Some individuals experienced minor health issues, such as fungal infections, but the majority remained active and healthy. Environmental factors such as temperature, humidity, pH, dissolved oxygen, total dissolved solids, and light intensity were within ranges conducive to acclimatization. These findings suggest that the aquarium at the TLC is quite effective as an ex situ conservation facility in supporting the adaptation process of D. subplana outside its natural habitat.
creator: Meiredo Marliansa
creator: Bhakti Karyadi
creator: Deni Parlindungan
creator: Aceng Ruyani
creator: Euis Nursa'adah
rights: Copyright (c) 2025 Meiredo Marliansa, Bhakti Karyadi, Deni Parlindungan, Aceng Ruyani, Euis Nursa'adah
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 261
ending page: 274
doi: 10.31932/jpbio.v10i2.4953

Climate change literacy ability profile in junior high school students in ogan ilir regency
type: item
description: Climate change is a global issue that requires awareness and understanding among younger generations to support sustainable environmental actions. This study aims to analyze the profile of climate change literacy among junior high school students (SMP) in Ogan Ilir Regency by considering school accreditation levels A, B, and C. The research employed a quantitative descriptive method using purposive sampling, involving 184 ninth-grade students from three schools. The instrument consisted of 40 items covering three indicators: knowledge, attitude, and competence, with Cronbach’s alpha reliability scores of 0.548, 0.900, and 0.511, respectively. Instrument validity testing showed adequate results, with a KMO value of 0.863, SRMR of 0.072, and GFI of 0.990. The findings indicate that 46% of students were in the moderate category of climate change literacy. The attitude indicator achieved the highest percentage at 48%, reflecting a good level of awareness and concern toward climate change issues. However, school accreditation does not necessarily determine literacy level, as evidenced by SMP C (Accreditation C), which recorded the highest percentage in the high category overall. These results imply the need to strengthen environmentally-based curricula and implement contextual learning approaches to enhance students’ climate change literacy.
creator: May Liza Anggraini
creator: Elvira Destiansari
creator: Meilinda Meilinda
rights: Copyright (c) 2025 May Liza Anggraini, Elvira Destiansari, Meilinda Meilinda
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 275
ending page: 289
doi: 10.31932/jpbio.v10i2.5097

Factors influencing green product purchase of adiwiyata and non-adiwiyata high school students in south jakarta
type: item
description: The global environmental crisis, exacerbated by unsustainable consumption and escalating plastic waste, underscores the urgency of promoting green product purchasing practices. This study investigates the influence of green product knowledge (GPK), green awareness (GA), and green lifestyle (GL) on green product purchasing practices (PPGP) among Adiwiyata and non-Adiwiyata high school students in South Jakarta. Employing a quantitative approach with a survey method, data were collected from 204 students using a combination of essay-based and Likert-scale questionnaires. Multiple linear regression analysis revealed that GPK, GA, and GL jointly had a significant influence on PPGP, explaining 56.07% of the variance for Adiwiyata students and 36.21% for their non-Adiwiyata counterparts. Partially, only GA and GL significantly affected PPGP among Adiwiyata students, whereas only GL was significant for non-Adiwiyata students. GL emerged as the strongest predictor in both groups, with regression coefficients of 0.6436 and 0.5823, respectively. These findings highlight the importance of embedding behavioral components into environmental education by prioritizing green lifestyle development to foster sustainable consumer behavior among high school students.
creator: Cholifah Dunya
creator: Eny Supriyati Rosyidatun
creator: Solihin Solihin
rights: Copyright (c) 2025 Cholifah Dunya
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 290
ending page: 304
doi: 10.31932/jpbio.v10i2.5099

Supporting sustainable development goals 4 through ethnoscience: Development of a scientific magazine on madurese shrimp paste
type: item
description: Education that integrates local wisdom with scientific concepts is essential to create meaningful and contextual learning experiences. This study was aimed at developing and evaluating the feasibility of a scientific magazine based on the ethnoscience of Madurese shrimp paste as a biology learning medium for class X at Islamic Senior High School (MA). The method employed was the Research and Development (R&D) 4-D model (Define, Design, Develop, Disseminate). The validation process showed a very high level of feasibility, with an average score of 92.5% from two subject matter experts, 93% from two media experts, and 95% from a biology teacher. In addition, the limited trial involving 30 students resulted in an average feasibility score of 93.4%. The results indicated that the scientific magazine based on the ethnoscience of Madurese shrimp paste was highly feasible as an alternative teaching material, contextual, engaging, and supportive of biology learning within the Merdeka Curriculum. Furthermore, the product was considered to have strong potential to enhance scientific literacy and promote the preservation of local wisdom in education, especially in support of SDG 4.
creator: Nafilatun Nisak
creator: Linda Tri Antika
creator: Lukluk Ibana
rights: Copyright (c) 2025 Nafilatun Nisak, Linda Tri Antika, Lukluk Ibana
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 305
ending page: 317
doi: 10.31932/jpbio.v10i2.5102

Analysis of students’ science process skills in low plant botany online learning through telegram
type: item
description: Research that discusses and examines the role of Telegram in developing science process skills (SPS) in low plant botany (LPB) learning in depth is still minimal. Therefore, the research aims to determine the level of SPS of students in LPB online learning through Telegram. The research method used a quantitative descriptive study of students' SPS levels after LPB learning through Telegram. The research was conducted with 60 students, using a questionnaire as the research instrument, followed by quantitative analysis. Results showed that students had a deep understanding and an adequate level of SPS in LPB online learning through Telegram, as evidenced by the average SPS score of 72.91% in the high category. Thus, Telegram has been proven to help develop students' SPS in LPB online learning. The study provides implications for educators by utilizing Telegram as a platform for online learning methods to develop students' SPS skills.
creator: Yudhistian Yudhistian
creator: Tabitha Sri Hartati Wulandari
rights: Copyright (c) 2025 Yudhistian Yudhistian, Tabitha Sri Hartati Wulandari
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 318
ending page: 327
doi: 10.31932/jpbio.v10i2.5156

Integrating STEM education and socio-scientific issues: Impacts on student creativity
type: item
description: Creativity is an essential 21st-century skill that students must develop to solve real-life scientific and environmental problems. This study aims to determine the effect of the STEM-Socio Scientific Issues learning model on students' creativity in the concept of organic waste management. Students' creativity is measured based on four indicators according to Torrance, namely fluency, flexibility, originality, and elaboration. This study uses a quasi-experimental method with a non-equivalent control group design. The research subjects consist of two classes at Muhammadiyah High School in Sukabumi City. Data were collected through creativity tests in the form of pretest and posttest essay questions that had been validated and reliability-tested. The results showed that the STEM-SSI learning model significantly improved students' creativity, particularly in the aspects of fluency (N-Gain 0.4031), flexibility (0.3029), and originality (0.3017), with a moderate category. However, on the elaboration indicator, the control class achieved a slightly higher N-Gain score (0.3574) compared to the experimental class (0.3396), indicating that this aspect has not developed optimally in STEM-SSI learning. Nevertheless, since three out of four creativity indicators showed significant improvement in the experimental class, it can be concluded that STEM-SSI learning can enhance students' creativity, particularly in terms of fluency, flexibility, and originality.
creator: Nafa Navi'a Hayati
creator: Setiono Setiono
creator: Sistiana Windyariani
rights: Copyright (c) 2025 Nafa Navi'a Hayati
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 328
ending page: 340
doi: 10.31932/jpbio.v10i2.5207

Reduction lead metal in process marinating local food fish pendap using lemon juice (citrus limon)
type: item
description: Fish have the potential to be contaminated with heavy metals such as lead, which can originate from human activities, industry, and ports. The Bengkulu people have a traditional processing method that is believed to reduce heavy metal content in fish through the immersion of fish in Pendap dishes using water and lemon. This study aims to observe the reduction of heavy metal content in fish during the immersion process using water and lemon juice in Pendap dishes. The research was experimental with positive and negative results and analyzed using the SPSS ANOVA test to see significant differences between treatment groups. The fish used as samples included Tongkol Sarda orentalis, Kembung Rastrelliger kanagurta, Tuna Thunnus albacares, and Tenggiri Scomberomorus Lacepède. The parts of the fish that were analyzed included the muscles, liver, and gills. All samples were exposed to Pb(NO3)2 and divided into 3 treatment groups: 1 positive control, 2 precipitated with water, and 3 precipitated with lemon juice (Citrus limon). The samples were tested using the reagents NaOH, KI, NH4OH, and HCl. Based on the research results, it was found that the application of lemon juice produced significant results in reducing Pb levels, as indicated by the negative test results.
creator: Nora Ayudi Asti
creator: Henny Johan
creator: Deni Parlindungan
creator: Ariefa Primairyani
creator: Aprina Defianti
creator: Euis Nursaadah
rights: Copyright (c) 2025 nora ayudiasti, Henny Johan, Deni Parlindungan, Ariefa Primairyani, Aprina Defianti, Euis Nursaadah
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 341
ending page: 350
doi: 10.31932/jpbio.v10i2.5209

The influence of the discovery learning model with differentiated learning on the HOTS learning outcomes
type: item
description: Higher-Order Thinking Skills (HOTS) is an important competency of the 21st century; yet, HOTS among high school students in Indonesia remains relatively low. This study aims to determine the effect of the discovery learning model with differentiated learning on the HOTS learning outcomes of high school students on the subject of motion systems. The research method employed was quantitative, utilizing a quasi-experimental design. The sample consists of two class XI Moving Biology classes from SMAN 61 Jakarta, selected through cluster random sampling, each consisting of 30 students. The research instrument consisted of a HOTS test with 13 essay questions covering cognitive levels C4 (analyzing), C5 (evaluating), and C6 (creating), an observation sheet, and a learning style questionnaire. The research results showed that the average posttest score of the experimental class (67.53) was higher compared to the control class (55.30). The t-test on the gain score showed a significant difference between the two classes. This study concludes that the discovery learning model with a differentiated learning approach has a significant effect on the HOTS learning outcomes of high school students on the topic of motion systems.
creator: Daniar Setyo Rini
creator: Rusdi Rusdi
creator: Siti Nurlaela
rights: Copyright (c) 2025 Siti Nurlaela
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 351
ending page: 361
doi: 10.31932/jpbio.v10i2.5220

The effect of nearpod-assisted problem-based learning on environmental literacy
type: item
description: The lack of interactive and contextual learning models further weakens student engagement with environmental issues. This study aims to determine the effect of the Problem-Based Learning (PBL) model assisted by Nearpod on students' environmental literacy regarding climate change material in Grade 10 at SMA Negeri 10 Tasikmalaya. The research method used is a Quasi-Experimental design. The research population consists of 10 classes, with a purposive sampling method yielding a sample size of 2 classes, comprising an experimental class and a control class. The research instruments used tests and non-tests in the form of multiple-choice questions and questionnaires. The results of the ANCOVA test showed that the Nearpod-assisted PBL model had a significant partial effect on environmental literacy in the cognitive and affective indicators, with a significance value of 0.000, but did not show an effect on the behavioral indicator, which obtained a significance value of 0.874. Meanwhile, the simultaneous test results for the combined three environmental literacy indicators yielded a significance of 0.184 (p-value > 0.05), indicating that the implementation of the Nearpod-assisted PBL model has not yet been able to provide a comprehensive impact on environmental literacy among students at SMAN 10 Tasikmalaya.
creator: Rini Nurjanah
creator: Dita Agustian
creator: Mufti Ali
rights: Copyright (c) 2025 Rini Nurjanah, Dita Agustian, Mufti Ali
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 362
ending page: 370
doi: 10.31932/jpbio.v10i2.5250

The implementation of quantum learning strategies in ecology materials to enhance students' creative thinking skills
type: item
description: Creative thinking is a key 21st-century skill that must be fostered in science education, particularly in ecology, which demands conceptual understanding and contextual problem solving. However, classroom learning often relies on conventional methods that limit student engagement and creativity. This study examines the effect of quantum learning strategies on improving students’ creative thinking skills in ecology. Quantum learning was selected for its holistic approach that integrates emotional, intellectual, and social dimensions, potentially optimizing creative stimulation. The research employed a quasi-experimental design with a pretest–posttest control group. Participants were 10th-grade students at SMA Negeri 11 Medan, divided into experimental and control groups. Data were collected using ecology-based creative thinking tests. The findings revealed a significant improvement in the creative thinking abilities of students in the experimental group compared to those in the control group. These results demonstrate that quantum learning effectively enhances students’ creativity in ecological learning contexts. The study suggests that adopting quantum learning strategies can help educators design more engaging and innovative learning environments aligned with 21st-century educational demands.
creator: Adi Hartono
creator: Tri Mustika Sarjani
creator: Dini Fitria
creator: Nanda Fahira Sulaiman
rights: Copyright (c) 2025 ADI HARTONO, TRI MUSTIKA SARJANI, DINI FITRIA, NANDA FAHIRA SULAIMAN
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 371
ending page: 380
doi: 10.31932/jpbio.v10i2.5254

Community structure and exploitation pressure of giant clam in dedap island-abang waters batam
type: item
description: The community structure of Tridacnidae in the shallow coral waters of Dedap Island, Batam, has been analysed through a quantitative approach to reveal ecological dynamics and exploitation pressures from the surrounding communities. This study employed transect methods and ecological index analysis, including Shannon-Wiener diversity (H?), evenness (E), and Simpson's dominance (D) across five observation stations. The results indicate a high dominance of Tridacna crocea in locations with low exploitation pressure, whereas T. maxima and T. squamosa, which are still hunted by the local population, exhibit limited abundance. Pearson correlation revealed that dissolved oxygen (DO) has a strong positive relationship with H? and E, and a negative relationship with D, while temperature showed an inverse relationship. The integration of ecological analysis with socio-ecological factors revealed that anthropogenic exploitation pressures also shape community structure spatially. This study offers a holistic approach to the conservation management of Tridacnidae based on community participation and habitat zoning, while simultaneously addressing gaps in ecological and social research in the coral reef areas of western Indonesia.
creator: Yuria Liza Meilanda
creator: Yarsi Efendi
creator: Fenny Agustina
rights: Copyright (c) 2025 Yuria Liza Meilanda, Yarsi Efendi, Fenny Agustina
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 381
ending page: 399
doi: 10.31932/jpbio.v10i2.5257

Reducing academic burnout biology among boarding school students through LIVE (liveworksheet and videos)
type: item
description: Academic burnout is a common issue among boarding school students due to demanding routines and high academic pressure, particularly in Biology classes, which involve many abstract concepts. This study aimed to examine the effectiveness of using LiveWorksheet and instructional videos in reducing the level of academic burnout among boarding school students. A one-group pre–post test design with two post-intervention measurements was employed. The sample consisted of senior high school boarding students who participated in Biology learning activities using a combination of LiveWorksheet and instructional videos. Data were collected using an academic burnout instrument and analyzed with paired sample t-tests. The results showed a decrease in burnout scores from a mean of 31.67 before the intervention to 25.37 in the first post-test (p = 0.005) and 25.83 in the second post-test (p = 0.007). These findings indicate that the combination of LiveWorksheet and instructional videos effectively reduced burnout by enhancing learning engagement, lowering cognitive load, and strengthening self-efficacy. Further research is recommended to use a control group design and long-term measurements to examine the sustainability of the intervention’s effects.
creator: Ghina Rohmatulloh
creator: Mimin Nurjhani Kusumastuti
creator: Kusnadi Kusnadi
creator: Heti Pratiwi
rights: Copyright (c) 2025 Ghina Rohmatulloh
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 400
ending page: 407
doi: 10.31932/jpbio.v10i2.5272

Effectiveness of a project-based learning digital booklet developed with the 4D model
type: item
description: The role of education is very important in producing quality human resources, one of which is through improving the quality of biology learning in schools. This study aims to determine the effectiveness of digital booklet-based PjBL (Project-Based Learning) learning media on the developed motion system material. The low level of conceptual understanding among 11th-grade students at SMAN 2 Binjai is caused by conventional learning methods and the lack of interactive media. The research method applied is a development study (R&D) using the 4D model (Define, Design, Develop, and Disseminate). The effectiveness of the media is measured by comparing pretest and posttest results, which are then analyzed using the N-Gain formula. The research findings indicate that this digital booklet is quite effective in improving learning outcomes, conceptual understanding, and student mobility. The average N-Gain score was 0.73, which falls into the moderate category. Therefore, it is concluded that the developed booklet is effective in its use as an innovative learning solution in the classroom.
creator: Rizal Mukra
creator: Tazkia Khansa Putri Rahima
creator: Oriza Satipa
creator: Assyfa Syahra
creator: Adelia Br. Tarigan
creator: Putri Hijriyanti
rights: Copyright (c) 2025 Tazkia Khansa Putri Rahima
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 408
ending page: 417
doi: 10.31932/jpbio.v10i2.5361

Development of augmented reality-based flashcard learning media for teaching leaf types
type: item
description: The use of this medium is expected to help students better understand and retain information about leaves, thereby contributing to improved learning outcomes in biology as a whole. This study aims to develop an effective Augmented Reality (AR)-based flashcard learning medium for the topic of leaf types. The research employed a Research and Development (R&D) approach using the ADDIE model, which includes the stages of Analysis, Design, Development, Implementation, and Evaluation. The research subjects consisted of 25 biology education students who had completed the Plant Morphology course, selected through a purposive sampling technique. The validation results indicated that the AR flashcard learning medium demonstrated a very high level of feasibility. Media expert validation obtained a score of 89.3%, while material expert validation yielded a score of 90%, both categorized as “highly feasible.” Furthermore, the evaluation based on educators’ responses achieved a score of 100%, also classified as “highly feasible.” These findings suggest that the AR-based flashcard learning medium received highly positive responses and proved effective in enhancing students’ interest and motivation in learning plant morphology.
creator: Dwi Aninditya Siregar
creator: Nabilah Siregar
creator: Sari Rozi Wahyuni Nasution
creator: Hanifah Nur Nasution
rights: Copyright (c) 2025 dwi aninditya siregar
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 418
ending page: 429
doi: 10.31932/jpbio.v10i2.5434

The implementation of discovery learning to improve scientific literacy in biology learning of protists
type: item
description: This study highlights the importance of developing 21st-century competencies, particularly students’ abilities to explain scientific phenomena, design investigations, and interpret data and evidence. The topic of Protists was chosen due to its high diversity and complexity, which often makes it difficult for students to understand, requiring a learning strategy that emphasizes active exploration and concept discovery. The study aimed to examine the effect of the Discovery Learning model on students’ scientific literacy in biology. A quasi-experimental pretest–posttest control group design was employed with two purposively selected tenth-grade classes in a senior high school. The experimental class (n = 20) used the Discovery Learning model, while the control class (n = 20) received conventional instruction. A scientific literacy test based on PISA indicators was used as the instrument. Results showed the experimental class’s mean score increased from 36.33 to 83.33, with an N-Gain of 0.70 (high category). Normality (p > 0.05) and homogeneity (p > 0.05) assumptions were met, and One-Way ANOVA (p < 0.05) indicated a significant difference, suggesting that Discovery Learning effectively improves students’ scientific literacy through active exploration, data analysis, and concept discovery.
creator: Saiman Rosamsi
creator: Fitri Aryanti
rights: Copyright (c) 2025 Saiman Rosamsi, Fitri Aryanti
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 430
ending page: 436
doi: 10.31932/jpbio.v10i2.5464

Correlation of cognitive learning outcomes and creativity in PjBL with culturally responsive teaching
type: item
description: Learning in the 21st century emphasizes higher-order thinking skills, particularly creativity and cognitive ability. However, observations in biology learning indicate that students’ creative thinking and conceptual understanding remain low, especially in biotechnology material. This study aims to determine the correlation between cognitive learning outcomes and creativity through the implementation of the Project-Based Learning (PjBL) model with the Culturally Responsive Teaching (CRT) approach. The research employed a quantitative correlational design involving 72 students of Grade X at SMA Negeri 1 Depok, Sleman, Yogyakarta. Data on creativity were collected using a validated creativity rubric, while cognitive learning outcomes were obtained from post-test scores. Data were analyzed using the Pearson product-moment correlation test with SPSS. The results showed that there was no significant correlation between cognitive learning outcomes and creativity (r = 0.090; p = 0.455 > 0.05). This finding suggests that an increase in cognitive learning outcomes does not necessarily lead to an increase in creativity. Thus, although the implementation of the PjBL-CRT approach contributed positively to students’ learning experiences, cognitive and creative abilities tended to develop independently. These results suggest the need for further learning strategies that explicitly integrate creative processes into cognitive learning tasks in biotechnology education.
creator: Alfiah Putri Ramadhani
creator: Agung Wijaya Subiantoro
rights: Copyright (c) 2025 Firdaus Ansyar
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 437
ending page: 446
doi: 10.31932/jpbio.v10i2.5473

Climate change e-module based on socio-scientific issues teaching and learning: A validity and practicality study
type: item
description: Climate change education is a priority in Indonesia's national curriculum. One challenge in teaching climate change in biology classes is the limited availability of teaching materials that cover various socio-scientific issues. Further development of these teaching materials is needed. This study aimed to develop a Climate Change E-Module based on Socio-Scientific Issues Teaching and Learning (SSI-TL) and test its validity and practicality in the learning process. This Research and Development (R&D) study used the Lee & Owens model, which focuses on developing multimedia-based learning designs. The e-module was created using Google Sites. Subject matter experts, media experts, and biology education practitioners assessed the e-module's validity. Practicality was assessed based on student responses through one-to-one trials, small group trials, and field trials. The validity test results showed the e-module to be highly valid, with percentages of 93% from a subject matter expert, 94% from a media expert, and 96% from a biology education practitioner. Practicality test results showed a highly practical category, with percentages of 87% for the one-to-one trial, 92% for the small group trial, and 93% for the field trial. Thus, the Climate Change E-Module based on SSI-TL is valid and practical for use in biology education.
creator: Moh. Usamah
creator: Fatchur Rohman
creator: Mimien Henie Irawati Al Muhdhar
rights: Copyright (c) 2025 Moh. Usamah, Fatchur Rohman, Mimien Henie Irawati Al Muhdhar
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 447
ending page: 461
doi: 10.31932/jpbio.v10i2.5488

Problem-based learning quartet cards development assisted by QR code: Critical thinking on environmental change
type: item
description: Conventional biology learning methods make students less active during the learning process. The lack of interactive media also causes students to have difficulty understanding the material, resulting in low critical thinking skills. This study aims to develop a Problem-Based Learning (PBL) based quartet card media assisted by QR codes on the topic of environmental change to improve students’ critical thinking skills. The research method used was Research and Development with the ADDIE model (Analysis, Design, Development, Implementation, and Evaluation). The research subjects were 34 students of class X4. The assessment instruments included needs analysis, expert validation sheets, teacher and student response questionnaires, and pretest–posttest evaluations. The results showed that the PBL-based quartet cards assisted by QR codes obtained a validity score of 89% from media experts and 98% from material experts. The quartet cards were also categorized as very practical based on students' responses score of 90% and teachers' responses score of 93%. The N-Gain test obtained a score of 0.76, with a percentage of 76%, which falls into the high category. Therefore, the Problem-Based Learning (PBL) based quartet card media assisted by QR codes is declared valid, practical, and effective for supporting biology learning.
creator: Alfina Sukma Hayati Damanik
creator: Rahmadina Rahmadina
rights: Copyright (c) 2025 Alfina Sukma Hayati Damanik
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 462
ending page: 477
doi: 10.31932/jpbio.v10i2.5515

Exploration of lactic acid bacteria from husk and black rice (Oryza sativa L) of toraja origin
type: item
description: Lactic acid (LAB) is a bacteria involved in food fermentation and can be used as a probiotic to provide health benefits for those who consume it. This research aims to bring innovation and added value, and improve the community's utilization of probiotics (LAB) from husks and black rice from Toraja. The method used is collecting and processing samples, isolation and purification of LAB, microscopic and macroscopic identification, testing the activity/potential of LAB (Kirby Bauer), and molecular identification of 16S rRNA (sequencing). This study has confirmed that fermented rice has Lactococcus taiwanensis type lactic acid bacteria, while fermented husk is not a type of lactic acid bacteria. Lactococcus taiwanensis bacteria have probiotic potential and can produce antibacterial substances.
creator: Saldi Hapiwaty
creator: Tuti Handayani Zainal
creator: Hamdayani Lance Abidin
creator: Yuri Pratiwi Utami
rights: Copyright (c) 2025 Saldi Hapiwaty
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 478
ending page: 487
doi: 10.31932/jpbio.v10i2.5529

E-module practicum of plant tissue culture with augmented reality to enhance lateral thinking and digital literacy
type: item
description: Plant tissue culture is a course requiring both theoretical understanding and practical skills in observation and analysis. Observations at the plant tissue culture laboratory of PGRI Pontianak University indicated that many students struggled to analyze culture stages, calculate appropriate media composition, and faced equipment limitations. This study aimed to determine the validity, practicality, and effectiveness of an augmented reality-assisted e-practicum module. The research employed an R&D approach with the ADDIE model. Data were collected through validation sheets, practical questionnaires, and lateral thinking skill tests for prospective teacher students. Data analysis involved calculating validation scores, questionnaire results, and N-Gain scores. The findings showed that the AR-assisted e-practicum module was valid in material aspects with an average score of 80 and very valid in media aspects with a score of 82. Student responses indicated the module was very practical, with an average score of 82%. The effectiveness test yielded an N-Gain score of 0.71, categorized as high. These results demonstrate that the AR-assisted e-practicum module can enhance students’ lateral thinking skills and digital literacy, suggesting its potential as a valuable teaching tool for future use.
creator: Tesa Manisa
creator: Nawawi Nawawi
rights: Copyright (c) 2025 Tesa Manisa
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 488
ending page: 501
doi: 10.31932/jpbio.v10i2.5531

Variation of morphological response of cayenne pepper cultivars to phenoxaprop-p-ethyl herbicide
type: item
description: In the cultivation of cayenne pepper (Capsicum frutescens L.), there is a problem with weeds. The use of herbicides for weed control can also trigger phytotoxic reactions in cultivated plants. The purpose of this study was to determine the morphological response of various cultivars of cayenne pepper to exposure to the Phenoxaprop-p-ethyl herbicide. This study employed a completely randomized design. The research data were analyzed using ANOVA and Kruskal-Wallis statistical tests. The study's results revealed a strong biphasic (hormesis) response pattern, where a dose of 55 g/L triggers growth, while a dose of 110 g/L causes phytotoxicity. .
creator: Nurul Rifqi Taqiya
creator: Edi Purwanto
creator: Suratman Suratman
rights: Copyright (c) 2025 Nurul Rifqi Taqiya
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 502
ending page: 512
doi: 10.31932/jpbio.v10i2.5544

Effect honje forest fruit juice administration on hemoglobin levels following mercury chloride treatment in mice
type: item
description: Exposure to mercury chloride (HgCl?) is known to induce oxidative stress, which negatively affects the hematological system, including a decrease in hemoglobin levels. This study aims to analyze the effect of honje fruit juice (Etlingera hemisphaerica), known for its high antioxidant content, on hemoglobin levels in mice (Mus musculus) exposed to mercury chloride. This study used an experimental method involving several groups of mice, namely group K1 (control), group K2 (given honje fruit juice), and group K3 (injected with mercury and given honje fruit juice). Hemoglobin levels were measured using a Sahli tube. The results showed that honje fruit juice significantly increased hemoglobin levels in mice exposed to mercury chloride compared to the control group. These findings indicate that the antioxidant content in honje fruit can help reduce the toxic effects of mercury chloride on the circulatory system. Therefore, honje fruit juice has the potential to be developed as a natural therapeutic agent in efforts to mitigate the toxic effects of mercury chloride.
creator: Aiza Sabina Putri Iskandar
creator: Aceng Ruyani
creator: Deni Parlindungan
creator: Henny Johan
creator: Afrizal Mayub
rights: Copyright (c) 2025 Aiza Sabina Putri Iskandar
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 513
ending page: 520
doi: 10.31932/jpbio.v10i2.5561

BioDiv-Web: Integrating structured project-based learning with citizen science to enhance critical thinking and environmental literacy
type: item
description: The escalating global biodiversity crisis underscores the critical need for effective educational strategies that promote environmental awareness and competencies. This research and development study addresses this imperative by designing and evaluating "BioDiv Web," an innovative e-learning platform for biodiversity education. The platform synergistically integrates the Structured Project-Based Learning (SPjBL) model with a Citizen Science Project (CSP) approach to create an authentic and scaffolded learning environment. Developed using the Lee and Owens model, the product was validated by experts and deemed highly valid (e.g., material validation: 100%) and practical (average score: 92%) based on trials with students. A quasi-experimental study involving 70 tenth-grade students was conducted to assess its effectiveness. The experimental group exhibited significantly higher learning gains compared to the control group. The results demonstrated moderate effectiveness, with normalized gain scores of 0.68 for critical thinking skills and 0.65 for environmental literacy. One-way ANCOVA analysis confirmed that these improvements were statistically significant (p < 0.001). The findings affirm that the BioDiv Web platform is a robust, practical, and effective digital tool for enhancing senior high school students' critical cognitive and affective learning outcomes in biodiversity education.
creator: Uswatul Wadhichatis Tsaniyah
creator: Mimien Henie Irawati Al Muhdhar
creator: Muntholib Muntholib
rights: Copyright (c) 2025 Uswatul Wadhichatis Tsaniyah
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 521
ending page: 539
doi: 10.31932/jpbio.v10i2.5603

Ethnobotany of spice plants among the community of emparu baru village, dedai district, sintang regency
type: item
description: The people of Emparu Baru Village still maintain local wisdom, including the traditional use of herbs and spices as cooking spices. However, this local wisdom is still passed down orally and has not been documented in writing. This study aims to identify and document the diversity of spice plants used by the Dayak Desa community in Emparu Baru Village, Dedai District, Sintang Regency. The research employed a qualitative descriptive method with an ethnobotanical approach. The results show that the Dayak Desa people possess extensive knowledge of various types of local spice plants, including rhizomes, leaves, tubers, fruits, seeds, and others. This traditional knowledge not only plays an important role in maintaining the resilience of local species but also holds great potential to be developed as a valuable source of information for efforts to conserve plant biodiversity. Based on the ethnobotanical study of spice plants, it can be concluded that there are 42 plant species from 25 families used as spices for cooking. These spices are derived from various plant parts, including fruits, stems, seeds, flowers, leaves, young leaves, bark, rhizomes, tubers, bulbs, and shoots.
creator: Benediktus Ege
creator: Yakobus Bustami
creator: Florentina Rahayu Esti Wahyuni
creator: Monica Kristiana Nova
rights: Copyright (c) 2025 Benediktus Ege
date: 2025-11-29
publication date: 2025-11-29
volume: 10
number: 2
starting page: 540
ending page: 552
doi: 10.31932/jpbio.v10i2.5628